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225  structures 8971  species 0  interactions 731832  sequences 4885  architectures

Clan: MFS (CL0015)


Major Facilitator Superfamily Add an annotation

The major facilitator superfamily (MFS) is one of the two largest families of membrane transporters found on Earth [1]. It is present ubiquitously in bacteria, archaea, and eukarya and includes members that can function by solute uniport, solute/cation symport, solute/cation antiport and/or solute/solute antiport with inwardly and/or outwardly directed polarity [1]. All permeases of the MFS possess either 12 or 14 transmembrane helices [1].

This clan contains 26 families and the total number of domains in the clan is 731832. The clan was built by A Bateman.

Literature references

  1. Pao SS, Paulsen IT, Saier MH Jr; , Microbiol Mol Biol Rev 1998;62:1-34.: Major facilitator superfamily. PUBMED:9529885 EPMC:9529885


This clan contains the following 26 member families:

Acatn ATG22 BT1 CLN3 DUF5690 Folate_carrier FPN1 LacY_symp MFS_1 MFS_1_like MFS_2 MFS_3 MFS_4 MFS_5 MFS_Mycoplasma Nodulin-like Nuc_H_symport Nucleoside_tran OATP PTR2 PUCC Sugar_tr TLC TRI12 UNC-93 UVB_sens_prot

Domain organisation

Below is a listing of the unique domain organisations or architectures from this clan. More...

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The table below shows the number of occurrences of each domain throughout the sequence database. More...

Pfam family Num. domains Alignment
MFS_1 (PF07690) 477899 (65.3%) View
Sugar_tr (PF00083) 129815 (17.7%) View
PTR2 (PF00854) 30082 (4.1%) View
MFS_2 (PF13347) 14089 (1.9%) View
MFS_3 (PF05977) 13707 (1.9%) View
OATP (PF03137) 7313 (1.0%) View
ATG22 (PF11700) 6858 (0.9%) View
Nucleoside_tran (PF01733) 5784 (0.8%) View
BT1 (PF03092) 5714 (0.8%) View
MFS_1_like (PF12832) 5211 (0.7%) View
UNC-93 (PF05978) 5209 (0.7%) View
Acatn (PF13000) 4633 (0.6%) View
Nodulin-like (PF06813) 4336 (0.6%) View
UVB_sens_prot (PF04884) 3293 (0.4%) View
Pfam family Num. domains Alignment
FPN1 (PF06963) 2502 (0.3%) View
TRI12 (PF06609) 2439 (0.3%) View
Folate_carrier (PF01770) 2314 (0.3%) View
CLN3 (PF02487) 2303 (0.3%) View
MFS_5 (PF05631) 2044 (0.3%) View
MFS_4 (PF06779) 1791 (0.2%) View
Nuc_H_symport (PF03825) 1381 (0.2%) View
TLC (PF03219) 1233 (0.2%) View
PUCC (PF03209) 998 (0.1%) View
DUF5690 (PF18943) 425 (0.1%) View
LacY_symp (PF01306) 413 (0.1%) View
MFS_Mycoplasma (PF07672) 46 (0.0%) View
Total: 26 Total: 731832 Clan alignment

Please note: Clan alignments can be very large and can cause problems for some browsers. Read the note above before viewing.

Family relationships

This diagram shows the relationships between members of this clan. More...

Species distribution

Tree controls


This tree shows the occurrence of the domains in this clan across different species. More...



For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the MSD group, to allow us to map Pfam domains onto UniProt three-dimensional structures. The table below shows the mapping between the Pfam families in this clan, the corresponding UniProt entries, and the region of the three-dimensional structures that are available for that sequence.

Loading structure mapping...