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1565  structures 9353  species 0  interactions 357755  sequences 3381  architectures

Clan: HAD (CL0137)


HAD superfamily Add an annotation

This clan represents the haloacid dehalogenase (HAD) superfamily that includes a diverse range of enzymes that use an asp carboxylate as a nucleophile [1].

This clan contains 25 families and the total number of domains in the clan is 357755. The clan was built by A Bateman.

Literature references

  1. Koonin EV, Tatusov RL; , J Mol Biol 1994;244:125-132.: Computer analysis of bacterial haloacid dehalogenases defines a large superfamily of hydrolases with diverse specificity. Application of an iterative approach to database search. PUBMED:7966317 EPMC:7966317
  2. Aravind L, Galperin MY, Koonin EV; , Trends Biochem Sci 1998;23:127-129.: The catalytic domain of the P-type ATPase has the haloacid dehalogenase fold. PUBMED:9584613 EPMC:9584613


This clan contains the following 25 member families:

5_nucleotid Acid_phosphat_B Acid_PPase APP1_cat Cation_ATPase DHH DUF2608 DUF705 HAD HAD_2 Hydrolase Hydrolase_3 Hydrolase_6 Hydrolase_like LNS2 NIF NT5C PGP_phosphatase PhoLip_ATPase_C PMM PNK3P Put_Phosphatase S6PP Trehalose_PPase UMPH-1

External database links

Domain organisation

Below is a listing of the unique domain organisations or architectures from this clan. More...

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The table below shows the number of occurrences of each domain throughout the sequence database. More...

Pfam family Num. domains Alignment
Hydrolase (PF00702) 77747 (21.7%) View
HAD_2 (PF13419) 67281 (18.8%) View
Cation_ATPase (PF13246) 33826 (9.5%) View
Hydrolase_3 (PF08282) 29841 (8.3%) View
Hydrolase_like (PF13242) 19507 (5.5%) View
PhoLip_ATPase_C (PF16212) 18992 (5.3%) View
HAD (PF12710) 17784 (5.0%) View
DHH (PF01368) 17271 (4.8%) View
NIF (PF03031) 16406 (4.6%) View
Hydrolase_6 (PF13344) 13521 (3.8%) View
Trehalose_PPase (PF02358) 10360 (2.9%) View
5_nucleotid (PF05761) 5547 (1.6%) View
LNS2 (PF08235) 4317 (1.2%) View
Pfam family Num. domains Alignment
Acid_phosphat_B (PF03767) 3681 (1.0%) View
APP1_cat (PF09949) 3173 (0.9%) View
PMM (PF03332) 2994 (0.8%) View
S6PP (PF05116) 2946 (0.8%) View
PNK3P (PF08645) 2772 (0.8%) View
NT5C (PF06941) 2213 (0.6%) View
Put_Phosphatase (PF06888) 2164 (0.6%) View
UMPH-1 (PF05822) 1965 (0.5%) View
Acid_PPase (PF12689) 1823 (0.5%) View
PGP_phosphatase (PF09419) 1443 (0.4%) View
DUF2608 (PF11019) 133 (0.0%) View
DUF705 (PF05152) 48 (0.0%) View
Total: 25 Total: 357755 Clan alignment

Please note: Clan alignments can be very large and can cause problems for some browsers. Read the note above before viewing.

Family relationships

This diagram shows the relationships between members of this clan. More...

Species distribution

Tree controls


This tree shows the occurrence of the domains in this clan across different species. More...



For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the MSD group, to allow us to map Pfam domains onto UniProt three-dimensional structures. The table below shows the mapping between the Pfam families in this clan, the corresponding UniProt entries, and the region of the three-dimensional structures that are available for that sequence.

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