Powering down the Pfam website
On October 5th, we began redirecting traffic from Pfam (pfam.xfam.org) to InterPro (www.ebi.ac.uk/interpro). The Pfam website will remain available at pfam-legacy.xfam.org until January 2023, when it will be decommissioned. You can read more about the sunset period in our blog post.

Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
0  structures 5531  species 0  interactions 13949  sequences 125  architectures

Clan: O-anti_assembly (CL0499)


O-antigen assembly enzyme superfamily Add an annotation

This superfamily is characterised by a group of bacterial proteins that lie in the membrane. The families are all enzymes involved in the assembly of O-antigen lipopolysaccharide, and include O-antigen flippase, polymerase and ligase, the flippase being the putative transporter [1].

This clan contains 5 families and the total number of domains in the clan is 13949. The clan was built by P Coggill.

Literature references

  1. Bonfil DJ, Ronen-Tarazi M, Sultemeyer D, Lieman-Hurwitz J, Schatz D, Kaplan A;, FEBS Lett. 1998;430:236-240.: A putative HCO3- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942. PUBMED:9688546 EPMC:9688546
  2. Marolda CL, Tatar LD, Alaimo C, Aebi M, Valvano MA;, J Bacteriol. 2006;188:5124-5135.: Interplay of the Wzx translocase and the corresponding polymerase and chain length regulator proteins in the translocation and periplasmic assembly of lipopolysaccharide o antigen. PUBMED:16816184 EPMC:16816184
  3. Fratamico PM, Yan X, Liu Y, DebRoy C, Byrne B, Monaghan A, Fanning S, Bolton D;, Can J Microbiol. 2010;56:308-316.: Escherichia coli serogroup O2 and O28ac O-antigen gene cluster sequences and detection of pathogenic E. coli O2 and O28ac by PCR. PUBMED:20453897 EPMC:20453897


This clan contains the following 5 member families:

O-ag_pol_Wzy O-antigen_lig O_anti_polymase Wzy_C WzyE

Domain organisation

Below is a listing of the unique domain organisations or architectures from this clan. More...

Loading domain graphics...


The table below shows the number of occurrences of each domain throughout the sequence database. More...

Pfam family Num. domains Alignment
Wzy_C (PF04932) 13158 (94.3%) View
O-ag_pol_Wzy (PF14296) 290 (2.1%) View
O-antigen_lig (PF13425) 266 (1.9%) View
WzyE (PF06899) 149 (1.1%) View
O_anti_polymase (PF01901) 86 (0.6%) View
Total: 5 Total: 13949 Clan alignment

Please note: Clan alignments can be very large and can cause problems for some browsers. Read the note above before viewing.

Family relationships

This diagram shows the relationships between members of this clan. More...

Species distribution

Tree controls


This tree shows the occurrence of the domains in this clan across different species. More...