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24  structures 6044  species 0  interactions 9647  sequences 34  architectures

Clan: CNF1_YfiH (CL0663)


CNF1/YfiH-like putative cysteine hydrolases Add an annotation

Members of this clan are structurally similar. Families are a class of deamidases with a common fold consisting of three-layered alpha/beta/beta sandwich, wherein two mixed 5-stranded beta sheets are flanked by a layer of two alpha helices. In a shallow cavity at the top of the alpha/beta/beta sandwich, an invariant Cys-His pair forms a catalytic dyad that is required by the toxins for deamidation activity [1]. Deamidation is used by some bacterial virulance factors to modulate eukaryotic host cell signaling systems [2].

This clan contains 4 families and the total number of domains in the clan is 9647. The clan was built by S El-Gebali.

Literature references

  1. Chao X, Muff TJ, Park SY, Zhang S, Pollard AM, Ordal GW, Bilwes AM, Crane BR;, Cell. 2006;124:561-571.: A receptor-modifying deamidase in complex with a signaling phosphatase reveals reciprocal regulation. PUBMED:16469702 EPMC:16469702
  2. Washington EJ, Banfield MJ, Dangl JL;, Microbiol Mol Biol Rev. 2013;77:527-539.: What a difference a Dalton makes: bacterial virulence factors modulate eukaryotic host cell signaling systems via deamidation. PUBMED:24006474 EPMC:24006474


This clan contains the following 4 member families:

BLF1 CheD CNF1 Cu-oxidase_4

Domain organisation

Below is a listing of the unique domain organisations or architectures from this clan. More...

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The table below shows the number of occurrences of each domain throughout the sequence database. More...

Pfam family Num. domains Alignment
Cu-oxidase_4 (PF02578) 6621 (68.6%) View
CheD (PF03975) 2999 (31.1%) View
CNF1 (PF05785) 25 (0.3%) View
BLF1 (PF17752) 2 (0.0%) View
Total: 4 Total: 9647 Clan alignment

Please note: Clan alignments can be very large and can cause problems for some browsers. Read the note above before viewing.

Family relationships

This diagram shows the relationships between members of this clan. More...

Species distribution

Tree controls


This tree shows the occurrence of the domains in this clan across different species. More...



For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the MSD group, to allow us to map Pfam domains onto UniProt three-dimensional structures. The table below shows the mapping between the Pfam families in this clan, the corresponding UniProt entries, and the region of the three-dimensional structures that are available for that sequence.

Loading structure mapping...