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116  structures 1549  species 0  interactions 28840  sequences 408  architectures

Clan: ENTH_VHS (CL0009)


ENTH/ANTH/VHS superfamily Add an annotation

This clan includes the related ENTH and ANTH domains as well as the VHS domain. The ENTH domain is approximately 150 residues in length and is a solenoid of alpha-helices. The various ENTH domains have various lipid specificities but the key feature that distinguishes it functionally from ANTH domains is its ability to bend membranes. It does this by folding an additional N-terminal helix on lipid binding. The ANTH domain is approximately 300 residues in length and is a PtdIns(4,5)P2 binding domain. It has no membrane bending properties. The VHS (Vps-27, Hrs and STAM) domain is a 140 residue long domain present in the very NH2-terminus of at least 60 proteins. Based on their functional characteristics and on recent data on the involvement of VHS in cargo recognition in trans-Golgi, VHS domains are considered to have a general membrane targeting/cargo recognition role in vesicular trafficking [5].

This clan contains 3 families and the total number of domains in the clan is 28840. The clan was built by A Bateman and H McMahon.

Literature references

  1. Ford MG, Mills IG, Peter BJ, Vallis Y, Praefcke GJ, Evans PR, McMahon HT; , Nature 2002;419:361-366.: Curvature of clathrin-coated pits driven by epsin. PUBMED:12353027 EPMC:12353027
  2. Mills IG, Praefcke GJ, Vallis Y, Peter BJ, Olesen LE, Gallop JL, Butler PJ, Evans PR, McMahon HT; , J Cell Biol 2003;160:213-222.: EpsinR: an AP1/clathrin interacting protein involved in vesicle trafficking. PUBMED:12538641 EPMC:12538641
  3. Stahelin RV, Long F, Peter BJ, Murray D, De Camilli P, McMahon HT, Cho W; , J Biol Chem 2003;278:28993-28999.: Contrasting membrane interaction mechanisms of AP180 N-terminal homology (ANTH) and epsin N-terminal homology (ENTH) domains. PUBMED:12740367 EPMC:12740367
  4. Ford MG, Pearse BM, Higgins MK, Vallis Y, Owen DJ, Gibson A, Hopkins CR, Evans PR, McMahon HT; , Science 2001;291:1051-1055.: Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes. PUBMED:11161218 EPMC:11161218
  5. Lohi O, Poussu A, Mao Y, Quiocho F, Lehto VP; , FEBS Lett 2002;513:19-23.: VHS domain -- a longshoreman of vesicle lines. PUBMED:11911875 EPMC:11911875


This clan contains the following 3 member families:


Domain organisation

Below is a listing of the unique domain organisations or architectures from this clan. More...

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The table below shows the number of occurrences of each domain throughout the sequence database. More...

Pfam family Num. domains Alignment
VHS (PF00790) 11292 (39.2%) View
ANTH (PF07651) 10874 (37.7%) View
ENTH (PF01417) 6674 (23.1%) View
Total: 3 Total: 28840 Clan alignment

Please note: Clan alignments can be very large and can cause problems for some browsers. Read the note above before viewing.

Family relationships

This diagram shows the relationships between members of this clan. More...

Species distribution

Tree controls


This tree shows the occurrence of the domains in this clan across different species. More...



For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the MSD group, to allow us to map Pfam domains onto UniProt three-dimensional structures. The table below shows the mapping between the Pfam families in this clan, the corresponding UniProt entries, and the region of the three-dimensional structures that are available for that sequence.

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