Powering down the Pfam website
On October 5th, we began redirecting traffic from Pfam (pfam.xfam.org) to InterPro (www.ebi.ac.uk/interpro). The Pfam website will remain available at pfam-legacy.xfam.org until January 2023, when it will be decommissioned. You can read more about the sunset period in our blog post.

Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
7  structures 4117  species 0  interactions 6622  sequences 86  architectures

Clan: NFACT_RNA-bind (CL0684)


NEMF/FbpA/Caliban/Tae2 RNA binding Add an annotation

This clan includes the RNA-binding domains found in the mammalian NEMF, drosophila caliban, yeast Tae2, and bacterial FpbA-like proteins (NFACT). The RNA-binding domain associated with this family is also observed in distinct architectural contexts in other proteins across phylogenetically diverse prokaryotes.It is found in two eukaryotic gene contexts: fused to the NFACT-N and NFACT-C domains in the NFACT protein involved in the ribosomal quality control pathway which contributes to CAT-tailing and as a standalone domain. In bacteria, it is mostly found fused to the ThiI domain as a variant of the canonical tRNA 4-thiouridylation pathway [1].

This clan contains 2 families and the total number of domains in the clan is 6622. The clan was built by S El-Gebali.

Literature references

  1. Burroughs AM, Aravind L;, RNA Biol. 2014;11:360-372.: A highly conserved family of domains related to the DNA-glycosylase fold helps predict multiple novel pathways for RNA modifications. PUBMED:24646681 EPMC:24646681


This clan contains the following 2 member families:


Domain organisation

Below is a listing of the unique domain organisations or architectures from this clan. More...

Loading domain graphics...


The table below shows the number of occurrences of each domain throughout the sequence database. More...

Pfam family Num. domains Alignment
NFACT-R_1 (PF05670) 6092 (92.0%) View
NFACT-R_2 (PF18297) 530 (8.0%) View
Total: 2 Total: 6622 Clan alignment

Please note: Clan alignments can be very large and can cause problems for some browsers. Read the note above before viewing.

Family relationships

This diagram shows the relationships between members of this clan. More...

Species distribution

Tree controls


This tree shows the occurrence of the domains in this clan across different species. More...



For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the MSD group, to allow us to map Pfam domains onto UniProt three-dimensional structures. The table below shows the mapping between the Pfam families in this clan, the corresponding UniProt entries, and the region of the three-dimensional structures that are available for that sequence.

Loading structure mapping...