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4  structures 743  species 0  interactions 5917  sequences 106  architectures

Clan: Nckap1_CYFIP1-2 (CL0715)


Nckap1/CYFIP 1/2 superfamily Add an annotation

This clan includes components of the WASH and WAVE complexes which are structurally similar and regulate actin cytoskeletal dynamics through the control of Arp2-3 complex-mediated actin assembly [1,2,3]. WAVE is found in all eukaryotic kingdoms and plays a crucial role in many cellular processes including adhesion, migration, division and fusion [1]. In animals, WAVE proteins are important for a wide range of processes such as embryogenesis, neuron morphogenesis and plasticity, immune cell activation and chemotaxis, and cancer invasion and metastasis [1]. WASH, conserved from simple eukaryotes to human, localizes to early endosomal subdomains and regulates endocytic vesicle scission in an Arp2-3-dependent manner [3]. Including in this clan are Cytoplasmic FMR1-interacting proteins CYFIP1 and CYFIP2, Nck-associated protein 1 (Nckap1) from WAVE complex and WASH complex subunit 7 (KIAA1033).

This clan contains 3 families and the total number of domains in the clan is 5917. The clan was built by S Chuguransky.

Literature references

  1. Chen B, Brinkmann K, Chen Z, Pak CW, Liao Y, Shi S, Henry L, Grishin NV, Bogdan S, Rosen MK;, Cell. 2014;156:195-207.: The WAVE regulatory complex links diverse receptors to the actin cytoskeleton. PUBMED:24439376 EPMC:24439376
  2. Chen Z, Borek D, Padrick SB, Gomez TS, Metlagel Z, Ismail AM, Umetani J, Billadeau DD, Otwinowski Z, Rosen MK;, Nature. 2010;468:533-538.: Structure and control of the actin regulatory WAVE complex. PUBMED:21107423 EPMC:21107423
  3. Jia D, Gomez TS, Metlagel Z, Umetani J, Otwinowski Z, Rosen MK, Billadeau DD;, Proc Natl Acad Sci U S A. 2010;107:10442-10447.: WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes. PUBMED:20498093 EPMC:20498093


This clan contains the following 3 member families:

FragX_IP Nckap1 WASH-4_N

Domain organisation

Below is a listing of the unique domain organisations or architectures from this clan. More...

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The table below shows the number of occurrences of each domain throughout the sequence database. More...

Pfam family Num. domains Alignment
Nckap1 (PF09735) 2651 (44.8%) View
FragX_IP (PF05994) 2401 (40.6%) View
WASH-4_N (PF14745) 865 (14.6%) View
Total: 3 Total: 5917 Clan alignment

Please note: Clan alignments can be very large and can cause problems for some browsers. Read the note above before viewing.

Family relationships

This diagram shows the relationships between members of this clan. More...

Species distribution

Tree controls


This tree shows the occurrence of the domains in this clan across different species. More...



For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the MSD group, to allow us to map Pfam domains onto UniProt three-dimensional structures. The table below shows the mapping between the Pfam families in this clan, the corresponding UniProt entries, and the region of the three-dimensional structures that are available for that sequence.

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