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1  structure 775  species 0  interactions 2236  sequences 431  architectures

Family: ASH (PF15780)

Summary: Abnormal spindle-like microcephaly-assoc'd, ASPM-SPD-2-Hydin

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Abnormal spindle-like microcephaly-assoc'd, ASPM-SPD-2-Hydin Provide feedback

The ASH domain or N-terminal domain of abnormal spindle-like microcephaly-associated protein are found in proteins associated with cilia, flagella, the centrosome and the Golgi complex. The domain is also found in Hydin and OCRL whose deficiencies are associated with hydrocephalus and Lowe oculocerebrorenal syndrome (OCRL), respectively. The fact that Human ASPM protein carries an ASH domain indicates possible roles for ASPM in sperm flagellar or in ependymal cells' cilia. The presence of ASH in centrosomal and ciliary proteins indicates that ASPM may possess roles not only in mitotic spindle regulation, but also in ciliary and flagellar function [1].

Literature references

  1. Ponting CP;, Bioinformatics. 2006;22:1031-1035.: A novel domain suggests a ciliary function for ASPM, a brain size determining gene. PUBMED:16443634 EPMC:16443634


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR031549

The ASH (ASPM-SPD-2-Hydin) domain or N-terminal domain of abnormal spindle-like microcephaly-associated protein (ASPM) is found in proteins associated with cilia, flagella, the centrosome and the Golgi complex [ PUBMED:25018876 , PUBMED:15068791 ]. The domain is also found in Hydin and OCRL whose deficiencies are associated with hydrocephalus and Lowe oculocerebrorenal syndrome (OCRL), respectively. The presence of ASH in centrosomal and ciliary proteins indicates that ASPM may possess roles not only in mitotic spindle regulation, but also in ciliary and flagellar function [ PUBMED:16443634 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan PapD-like (CL0556), which has the following description:

This superfamily is characterised by proteins in families involved in ciliary or flagellar function. The families may be acting as chaperones.

The clan contains the following 8 members:

ASH DUF1573 EcpB_C Motile_Sperm PapD-like PapD_N Peptidase_M60_C TMEM131_like_N

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(32)
Full
(2236)
Representative proteomes UniProt
(7519)
RP15
(416)
RP35
(1029)
RP55
(2018)
RP75
(2730)
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PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(32)
Full
(2236)
Representative proteomes UniProt
(7519)
RP15
(416)
RP35
(1029)
RP55
(2018)
RP75
(2730)
Alignment:
Format:
Order:
Sequence:
Gaps:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(32)
Full
(2236)
Representative proteomes UniProt
(7519)
RP15
(416)
RP35
(1029)
RP55
(2018)
RP75
(2730)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Ponting C [1]
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Coggill P
Number in seed: 32
Number in full: 2236
Average length of the domain: 94.8 aa
Average identity of full alignment: 20 %
Average coverage of the sequence by the domain: 5.83 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.0 27.0
Noise cut-off 26.9 26.9
Model length: 98
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the ASH domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
F1P4W9 View 3D Structure Click here
Q54NE5 View 3D Structure Click here
Q9VC45 View 3D Structure Click here