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15  structures 1444  species 0  interactions 6516  sequences 92  architectures

Family: BET (PF17035)

Summary: Bromodomain extra-terminal - transcription regulation

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Bromodomain extra-terminal - transcription regulation Provide feedback

The BET, or bromodomain extra-terminal domain, is found on bromodomain proteins that play key roles in development, cancer progression and virus-host pathogenesis. It interacts with NSD3, JMJD6, CHD4, GLTSCR1, and ATAD5 all of which are shown to impart a pTEFb-independent transcriptional activation function on the bromodomain proteins [1].

Literature references

  1. Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM;, Mol Cell Biol. 2011;31:2641-2652.: The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. PUBMED:21555454 EPMC:21555454

Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR027353

The bromodomain and extraterminal (BET) proteins are a class of transcriptional regulators whose members can be found in animals, plants and fungi. BET proteins are involved in diverse cellular phenomena such as meiosis, cell-cycle control, and homeosis and have been suggested to modulate chromatin structure and affect transcription via a sequence-independent mechanism. BET proteins are defined as having one (plants) or two (animals/yeast) bromodomains and an Extra Terminal (ET) domain. The ET domain consists of three separate regions, only one of which, the N-terminal ET (NET) domain is conserved in all BET proteins. The function of the NET domain is assumed to be protein binding [ PUBMED:7816623 , PUBMED:11487468 , PUBMED:12969431 , PUBMED:18815416 ].

The structure of the NET domain comprises three alpha-helices and a characteristic loop region of an irregular but well-defined structure. The NET structure has an acidic patch that forms a continuous ridge with a hydrophobic cleft. which may interact with other proteins and/or DNA [ PUBMED:18815416 ].

Some proteins known to contain a NET domain include:

  • Human RING3 (now designated Brd2)
  • Murine MCAP (now designated Brd4)
  • Drosophila Fsh
  • Yeast Bdf1 and Bdf2
  • Arabidopsis imbibition-inducible (IMB1), whichplays a role in abscisic acid (ABA) and phytochrome A (phyA) mediated responses of seed germination.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan BET (CL0665), which has the following description:

The BET (bromodomain extra-terminal) superfamily includes the BET domain as well as the AHD domain from AF-9.

The clan contains the following 2 members:



We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Representative proteomes UniProt
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

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Representative proteomes UniProt

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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: [1]
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Coggill P
Number in seed: 155
Number in full: 6516
Average length of the domain: 64.2 aa
Average identity of full alignment: 36 %
Average coverage of the sequence by the domain: 9.21 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 24.5 21.9
Trusted cut-off 24.5 21.9
Noise cut-off 24.4 21.8
Model length: 64
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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The tree shows the occurrence of this domain across different species. More...


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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the BET domain has been found. There are 15 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A044SEZ0 View 3D Structure Click here
A0A077ZBQ9 View 3D Structure Click here
A0A0A2V6P4 View 3D Structure Click here
A0A0D2EIQ7 View 3D Structure Click here
A0A0D2FH50 View 3D Structure Click here
A0A0K0ET63 View 3D Structure Click here
A0A0N7KCJ7 View 3D Structure Click here
A0A0N7KJQ5 View 3D Structure Click here
A0A0P0WF17 View 3D Structure Click here
A0A0R0EVJ9 View 3D Structure Click here
A0A0R0EVS6 View 3D Structure Click here
A0A0R0FEY3 View 3D Structure Click here
A0A0R0FMA3 View 3D Structure Click here
A0A0R0GAG4 View 3D Structure Click here
A0A0R0I9Z0 View 3D Structure Click here
A0A0R0JUQ7 View 3D Structure Click here
A0A158Q4F5 View 3D Structure Click here
A0A175VZ03 View 3D Structure Click here
A0A175WID9 View 3D Structure Click here
A0A1C1C8T6 View 3D Structure Click here
A0A1C1CI16 View 3D Structure Click here
A0A1D6H5K1 View 3D Structure Click here
A0A1D6HCG5 View 3D Structure Click here
A0A1D6JEQ3 View 3D Structure Click here
A0A1D6LET0 View 3D Structure Click here
A0A1D6LKQ1 View 3D Structure Click here
A0A1D6MFX8 View 3D Structure Click here
A0A1D6NR09 View 3D Structure Click here
A0A1D6P350 View 3D Structure Click here
A0A1D6PWP4 View 3D Structure Click here
A0A1D6QPE6 View 3D Structure Click here
A0A1D8PH31 View 3D Structure Click here
A0A1P6C1K5 View 3D Structure Click here
A0A2R8Q080 View 3D Structure Click here
A0A2R8RSS8 View 3D Structure Click here
A0A3P7DUC9 View 3D Structure Click here
A0A5K4EQL3 View 3D Structure Click here
A0A5K4ESD7 View 3D Structure Click here
A3A538 View 3D Structure Click here
A5PN48 View 3D Structure Click here