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12  structures 2629  species 0  interactions 4339  sequences 51  architectures

Family: ChaC (PF04752)

Summary: ChaC-like protein

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

ChaC-like protein Provide feedback

The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides [1, 2]. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.

Literature references

  1. Kumar A, Tikoo S, Maity S, Sengupta S, Sengupta S, Kaur A, Bachhawat AK;, EMBO Rep. 2012;13:1095-1101.: Mammalian proapoptotic factor ChaC1 and its homologues function as gamma-glutamyl cyclotransferases acting specifically on glutathione. PUBMED:23070364 EPMC:23070364

  2. Kumar S, Kaur A, Chattopadhyay B, Bachhawat AK;, Biochem J. 2015;468:73-85.: Defining the cytosolic pathway of glutathione degradation in Arabidopsis thaliana: role of the ChaC/GCG family of gamma-glutamyl cyclotransferases as glutathione-degrading enzymes and AtLAP1 as the Cys-Gly peptidase. PUBMED:25716890 EPMC:25716890


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR006840

The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides [ PUBMED:23070364 , PUBMED:25716890 ]. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan AIG2 (CL0278), which has the following description:

The structure consists of a five-stranded beta-barrel surrounded by two alpha-helices and a small beta-sheet. Conservation of residues in a hydrophilic cavity able to bind small ligands in some members suggests that this may also serve as an active site.

The clan contains the following 3 members:

AIG2_2 ChaC GGACT

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(9)
Full
(4339)
Representative proteomes UniProt
(13975)
RP15
(669)
RP35
(1971)
RP55
(3872)
RP75
(6304)
Jalview View  View  View  View  View  View  View 
HTML View  View           
PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(9)
Full
(4339)
Representative proteomes UniProt
(13975)
RP15
(669)
RP35
(1971)
RP55
(3872)
RP75
(6304)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(9)
Full
(4339)
Representative proteomes UniProt
(13975)
RP15
(669)
RP35
(1971)
RP55
(3872)
RP75
(6304)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_3722 (release 7.5)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Mifsud W
Number in seed: 9
Number in full: 4339
Average length of the domain: 166.1 aa
Average identity of full alignment: 34 %
Average coverage of the sequence by the domain: 77.56 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.0 21.0
Trusted cut-off 21.1 21.0
Noise cut-off 20.9 20.9
Model length: 181
Family (HMM) version: 15
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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The tree shows the occurrence of this domain across different species. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the ChaC domain has been found. There are 12 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A044UKW3 View 3D Structure Click here
A0A0D2GY47 View 3D Structure Click here
A0A0H3GZK1 View 3D Structure Click here
A0A0K0EMV4 View 3D Structure Click here
A0A0N4U1N5 View 3D Structure Click here
A0A175W7Y8 View 3D Structure Click here
A0A1C1CLG7 View 3D Structure Click here
A0A1D6FRW8 View 3D Structure Click here
A0A1D6GMP5 View 3D Structure Click here
A0A1D6I1E6 View 3D Structure Click here
A0A1D6J7P1 View 3D Structure Click here
A0A1D6KLZ6 View 3D Structure Click here
A0A1D6KP81 View 3D Structure Click here
A0A1D6KXZ9 View 3D Structure Click here
A0A1D6QBN8 View 3D Structure Click here
A0A1D6QP22 View 3D Structure Click here
A0A1D6QP23 View 3D Structure Click here
A0A1D8PL00 View 3D Structure Click here
A0A1P6BY29 View 3D Structure Click here
A0A3P7DCG8 View 3D Structure Click here
A4HZZ0 View 3D Structure Click here
A4HZZ1 View 3D Structure Click here
B3STU3 View 3D Structure Click here
B4FBP8 View 3D Structure Click here
C0NAS4 View 3D Structure Click here
C1HDG6 View 3D Structure Click here
C6SXG9 View 3D Structure Click here
I1JUP2 View 3D Structure Click here
I1K963 View 3D Structure Click here
P32656 View 3D Structure Click here
P39163 View 3D Structure Click here
P87305 View 3D Structure Click here
Q0IIH4 View 3D Structure Click here
Q32GY6 View 3D Structure Click here
Q4DQU0 View 3D Structure Click here
Q4DX06 View 3D Structure Click here
Q4KMJ1 View 3D Structure Click here
Q54LB6 View 3D Structure Click here
Q57YD2 View 3D Structure Click here
Q5SPB6 View 3D Structure Click here