Summary: ArgJ family
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This is the Wikipedia entry entitled "ArgJ protein family". More...
ArgJ protein family Edit Wikipedia article
ArgJ | |||||||||
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![]() ornithine acetyltransferase (orf6 gene product - clavulanic acid biosynthesis) from streptomyces clavuligerus | |||||||||
Identifiers | |||||||||
Symbol | ArgJ | ||||||||
Pfam | PF01960 | ||||||||
InterPro | IPR002813 | ||||||||
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In molecular biology, members of the ArgJ protein family are bifunctional protein that catalyses the first (EC 2.3.1.35) and fifth steps (EC 2.3.1.1) in arginine biosynthesis.[1] The structure has been determined for glutamate N-acetyltransferase 2 (ornithine acetyltransferase), an ArgJ-like protein from Streptomyces clavuligerus.[2]
References
- ^ Sakanyan V, Charlier D, Legrain C, Kochikyan A, Mett I, Pierard A, Glansdorff N (1993). "Primary structure, partial purification and regulation of key enzymes of the acetyl cycle of arginine biosynthesis in Bacillus stearothermophilus: dual function of ornithine acetyltransferase". J. Gen. Microbiol. 139 (3): 393–402. PMID 8473852.
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ignored (help)CS1 maint: multiple names: authors list (link) - ^ Elkins JM, Kershaw NJ, Schofield CJ (2005). "X-ray crystal structure of ornithine acetyltransferase from the clavulanic acid biosynthesis gene cluster". Biochem. J. 385 (Pt 2): 565–73. doi:10.1042/BJ20040814. PMC 1134730. PMID 15352873.
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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.
ArgJ family Provide feedback
Members of the ArgJ family catalyse the first EC:2.3.1.1 and fifth steps EC:2.3.1.35 in arginine biosynthesis.
Literature references
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Sakanyan V, Charlier D, Legrain C, Kochikyan A, Mett I, Pierard A, Glansdorff N; , J Gen Microbiol 1993;139:393-402.: Primary structure, partial purification and regulation of key enzymes of the acetyl cycle of arginine biosynthesis in Bacillus stearothermophilus: dual function of ornithine acetyltransferase. PUBMED:8473852 EPMC:8473852
Internal database links
SCOOP: | Peptidase_S58 |
This tab holds annotation information from the InterPro database.
InterPro entry IPR002813
ArgJ (also known as Ornithine acetyltransferase/OAT) is a bifunctional protein that catalyses the first EC and fifth steps EC in arginine biosynthesis [ PUBMED:8473852 ], coupling acetylation of glutamate with deacetylation of N-acetylornithine, which allows recycling of the acetyl group in the arginine biosynthetic pathway. The structure has been determined for glutamate N-acetyltransferase 2 (ornithine acetyltransferase; EC ), an ArgJ-like protein from Streptomyces clavuligerus [ PUBMED:15352873 ].
Members of this family may experience feedback inhibition by L-arginine. The active enzyme is a heterotetramer of two alpha and two beta chains, where the alpha and beta chains are the result of autocatalytic cleavage. OATs found in the clavulanic acid biosynthesis gene cluster catalyze the fifth step only, and may utilize acetyl acceptors other than glutamate [ PUBMED:15375131 , PUBMED:15352873 , PUBMED:12633501 , PUBMED:8473852 , PUBMED:4365537 , PUBMED:15937278 ].
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Molecular function | glutamate N-acetyltransferase activity (GO:0004358) |
Biological process | arginine biosynthetic process (GO:0006526) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan DmpA_ArgJ (CL0635), which has the following description:
This superfamily includes the ArgJ protein and members of peptidase family S58. According to SCOP these proteins have a fold which resembles but is unrelated to that of the NTN hydrolases.
The clan contains the following 5 members:
ArgJ Ceramidase_alk MoCoBD_1 MoCoBD_2 Peptidase_S58Alignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (648) |
Full (7005) |
Representative proteomes | UniProt (29963) |
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RP15 (1011) |
RP35 (3576) |
RP55 (7047) |
RP75 (11511) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
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Seed (648) |
Full (7005) |
Representative proteomes | UniProt (29963) |
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RP15 (1011) |
RP35 (3576) |
RP55 (7047) |
RP75 (11511) |
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Raw Stockholm | |||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Enright A |
Previous IDs: | none |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Enright A |
Number in seed: | 648 |
Number in full: | 7005 |
Average length of the domain: | 377.6 aa |
Average identity of full alignment: | 40 % |
Average coverage of the sequence by the domain: | 93.28 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 375 | ||||||||||||
Family (HMM) version: | 21 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the ArgJ domain has been found. There are 44 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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AlphaFold Structure Predictions
The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.