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57  structures 2532  species 0  interactions 7506  sequences 1009  architectures

Family: PA14 (PF07691)

Summary: PA14 domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

PA14 domain Provide feedback

This domain forms an insert in bacterial beta-glucosidases and is found in other glycosidases, glycosyltransferases, proteases, amidases, yeast adhesins, and bacterial toxins, including anthrax protective antigen (PA). The domain also occurs in a Dictyostelium prespore-cell-inducing factor Psi and in fibrocystin, the mammalian protein whose mutation leads to polycystic kidney and hepatic disease. The crystal structure of PA shows that this domain (named PA14 after its location in the PA20 pro-peptide) has a beta-barrel structure. The PA14 domain sequence suggests a binding function, rather than a catalytic role. The PA14 domain distribution is compatible with carbohydrate binding.

Literature references

  1. Rigden DJ, Mello LV, Galperin MY; , Trends Biochem Sci. 2004;29:335-339.: The PA14 domain, a conserved all-beta domain in bacterial toxins, enzymes, adhesins and signaling molecules. PUBMED:15236739 EPMC:15236739


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR011658

The PA14 domain forms an insert in bacterial beta-glucosidases, other glycosidases, glycosyltransferases, proteases, amidases, yeast adhesins and bacterial toxins, including anthrax protective antigen (PA). The domain also occurs in a Dictyostelium pre-spore cell-inducing factor Psi and in fibrocystin, the mammalian protein whose mutation leads to polycystic kidney and hepatic disease. The crystal structure of PA shows that this domain (named PA14 after its location in the PA20 pro-peptide) has a beta-barrel structure. The PA14 domain sequence suggests a binding function, rather than a catalytic role. The PA14 domain distribution is compatible with carbohydrate binding [ PUBMED:15236739 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan PA14 (CL0301), which has the following description:

This clan includes the PA14 domain and related families.

The clan contains the following 2 members:

GLEYA PA14

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(22)
Full
(7506)
Representative proteomes UniProt
(27524)
RP15
(1194)
RP35
(3358)
RP55
(6890)
RP75
(11417)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(22)
Full
(7506)
Representative proteomes UniProt
(27524)
RP15
(1194)
RP35
(3358)
RP55
(6890)
RP75
(11417)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(22)
Full
(7506)
Representative proteomes UniProt
(27524)
RP15
(1194)
RP35
(3358)
RP55
(6890)
RP75
(11417)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Rigden DJ, Mello LV, Galperin MY
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Rigden DJ , Mello LV , Galperin MY
Number in seed: 22
Number in full: 7506
Average length of the domain: 132.8 aa
Average identity of full alignment: 15 %
Average coverage of the sequence by the domain: 13.19 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 24.0 24.0
Trusted cut-off 24.0 24.0
Noise cut-off 23.9 23.9
Model length: 147
Family (HMM) version: 15
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the PA14 domain has been found. There are 57 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0D2DTE5 View 3D Structure Click here
A0A175WEF7 View 3D Structure Click here
A0A1C1CRB5 View 3D Structure Click here
A0A1D8PJB5 View 3D Structure Click here
A1CA51 View 3D Structure Click here
A1CUR8 View 3D Structure Click here
A1DFA8 View 3D Structure Click here
A1DNN8 View 3D Structure Click here
A1DPG0 View 3D Structure Click here
A2R989 View 3D Structure Click here
A3DC75 View 3D Structure Click here
B8NDE2 View 3D Structure Click here
B8NPL7 View 3D Structure Click here
D5EY15 View 3D Structure Click here
E7F204 View 3D Structure Click here
K0EGH0 View 3D Structure Click here
K0EZF8 View 3D Structure Click here
P0CI67 View 3D Structure Click here
P13423 View 3D Structure Click here
P32768 View 3D Structure Click here
P36170 View 3D Structure Click here
P38894 View 3D Structure Click here
P39712 View 3D Structure Click here
P49008 View 3D Structure Click here
Q0CAF5 View 3D Structure Click here
Q1ZXC5 View 3D Structure Click here
Q2U8Y5 View 3D Structure Click here
Q2U9M7 View 3D Structure Click here
Q4WA69 View 3D Structure Click here
Q4WL79 View 3D Structure Click here
Q4WLY1 View 3D Structure Click here
Q4WU49 View 3D Structure Click here
Q54B88 View 3D Structure Click here
Q54C31 View 3D Structure Click here
Q54C32 View 3D Structure Click here
Q54CH8 View 3D Structure Click here
Q54DV5 View 3D Structure Click here
Q54FD8 View 3D Structure Click here
Q54G85 View 3D Structure Click here
Q54I92 View 3D Structure Click here