Powering down the Pfam website
On October 5th, we began redirecting traffic from Pfam (pfam.xfam.org) to InterPro (www.ebi.ac.uk/interpro). The Pfam website will remain available at pfam-legacy.xfam.org until January 2023, when it will be decommissioned. You can read more about the sunset period in our blog post.

Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
18  structures 1282  species 0  interactions 1610  sequences 35  architectures

Family: ATG101 (PF07855)

Summary: Autophagy-related protein 101

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Autophagy-related protein 101". More...

Autophagy-related protein 101 Edit Wikipedia article


Alt text
Seen here is the structure of the spindle assembly checkpoint protein Mad2, predicted to be 95% similar to ATG101. This is the closest known estimate to the structure of the novel protein ATG101.

C12orf44 is a relatively unknown protein sequence on the 12th chromosome and the 44th frame. According to a paper discussing ATG101, which is just another alias for C12orf44, we learn that ATG is related to a class of autophagy related proteins. Autophagy is a major route by which cytoplasmic contents are delivered to the lysosome for degradation. When searching Google, using the search term “C12orf44” yielded 283,000 results, and when switched to Google scholar, it yielded no valid results. Two patents have been applied for when the term C12orf44 and ATG101 were searched, neither of which were valid results that actually regarded to the genes. As previously stated, ATG101, as well as FLJ11773 are the only known aliases of C12orf44. Two papers were found addressing ATG101 in pubmed. Both discussed how the function of ATG101 was still unknown, however this novel protein is shown to interact with ATG13, suggesting that it is somehow involved in macroautophagy and may be used to regulate other proteins such as ATG13. Both ATG13 and ATG 101 are stated to be ULK1-interacting proteins, which further suggests their involvement in macroautophagy in mammalian cells.

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Autophagy-related protein 101 Provide feedback

Atg101 is a critical autophagy factor that functions together with ULK, Atg13 and FIP200 [1, 2]. Atg101 has been shown to be a HORMA (Hop1, Rev7 and MAD2) domain protein with an open conformation [3].

Literature references

  1. Hosokawa N, Sasaki T, Iemura S, Natsume T, Hara T, Mizushima N;, Autophagy. 2009;5:973-979.: Atg101, a novel mammalian autophagy protein interacting with Atg13. PUBMED:19597335 EPMC:19597335

  2. Mercer CA, Kaliappan A, Dennis PB;, Autophagy. 2009;5:649-662.: A novel, human Atg13 binding protein, Atg101, interacts with ULK1 and is essential for macroautophagy. PUBMED:19287211 EPMC:19287211

  3. Suzuki H, Kaizuka T, Mizushima N, Noda NN;, Autophagy. 2015;11:2123-2124.: Open and closed HORMAs regulate autophagy initiation. PUBMED:26389686 EPMC:26389686

Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR012445

Atg101 is a critical autophagy factor that functions together with ULK, Atg13 and FIP200 [ PUBMED:19597335 , PUBMED:19287211 ]. In fission yeasts, it has a role in meiosis and sporulation [ PUBMED:16303567 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Pfam Clan

This family is a member of clan Mad2 (CL0651), which has the following description:

The clan contains the following 5 members:

ATG101 ATG13 bacHORMA_2 HORMA p31comet


We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Representative proteomes UniProt
Jalview View  View  View  View  View  View  View 
HTML View  View           
PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

Representative proteomes UniProt

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

Representative proteomes UniProt
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_9402 (release 14.0)
Previous IDs: DUF1649;
Type: Family
Sequence Ontology: SO:0100021
Author: Fenech M
Number in seed: 97
Number in full: 1610
Average length of the domain: 158.7 aa
Average identity of full alignment: 31 %
Average coverage of the sequence by the domain: 70.23 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 26.5 26.5
Trusted cut-off 27.0 26.5
Noise cut-off 26.3 26.2
Model length: 153
Family (HMM) version: 15
Download: download the raw HMM for this family

Species distribution

Sunburst controls


Weight segments by...

Change the size of the sunburst


Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls


The tree shows the occurrence of this domain across different species. More...


Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.


For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the ATG101 domain has been found. There are 18 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

Loading structure mapping...