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27  structures 251  species 0  interactions 930  sequences 117  architectures

Family: GLEYA (PF10528)

Summary: GLEYA domain

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

GLEYA domain Provide feedback

The GLEYA domain is related to lectin-like binding domains found in the S. cerevisiae Flo proteins and the C. glabrata Epa proteins [1]. It is a carbohydrate-binding domain that is found in fungal adhesins (also referred to as agglutinins or flocculins) [2]. Adhesins with a GLEYA domain possess a typical N-terminal signal peptide and a domain of conserved sequence repeats, but lack glycosylphosphatidylinositol (GPI) anchor attachment signals [3]. They contain a conserved motif G(M/L)(E/A/N/Q)YA, hence the name GLEYA. Based on sequence homology, it is suggested that the GLEYA domain would predominantly contain beta sheets [1]. The GLEYA domain is also found in S. pombe protein Q92344 thought to be a kinetochore portein (Sim4 complex subunit), however no direct evidence for kinetochore association has been found [4]. Furthermore, a global protein localisation study in S. pombe identified it as a secreted protein localized to the Golgi complex [5].

Literature references

  1. Linder T, Gustafsson CM; , Fungal Genet Biol. 2007; [Epub ahead of print]: Molecular phylogenetics of ascomycotal adhesins-A novel family of putative cell-surface adhesive proteins in fission yeasts. PUBMED:17870620 EPMC:17870620

  2. Andersson KM, Meerupati T, Levander F, Friman E, Ahren D, Tunlid A;, Appl Environ Microbiol. 2013;79:4993-5004.: Proteome of the nematode-trapping cells of the fungus Monacrosporium haptotylum. PUBMED:23770896 EPMC:23770896

  3. Liu MC, Yang CS, Yeh FL, Wei CH, Jane WN, Chung MC, Wang CS;, J Exp Bot. 2014;65:2023-2037.: A novel lily anther-specific gene encodes adhesin-like proteins associated with exine formation during anther development. PUBMED:24591055 EPMC:24591055

  4. Liu X, McLeod I, Anderson S, Yates JR 3rd, He X; , EMBO J. 2005;24:2919-2930.: Molecular analysis of kinetochore architecture in fission yeast. PUBMED:16079914 EPMC:16079914

  5. Sideri T, Rallis C, Bitton DA, Lages BM, Suo F, Rodriguez-Lopez M, Du LL, Bahler J;, G3 (Bethesda). 2014;5:145-155.: Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence. PUBMED:25452419 EPMC:25452419


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR018871

The GLEYA domain is related to lectin-like binding domains found in the Saccharomyces cerevisiae Flo proteins and the Candida glabrata Epa proteins [ PUBMED:17870620 ]. It is a carbohydrate-binding domain that is found in fungal adhesins (also referred to as agglutinins or flocculins) [ PUBMED:23770896 ]. Adhesins with a GLEYA domain possess a typical N-terminal signal peptide and a domain of conserved sequence repeats, but lack glycosylphosphatidylinositol (GPI) anchor attachment signals [ PUBMED:24591055 ]. They contain a conserved motif G(M/L)(E/A/N/Q)YA, hence the name GLEYA. Based on sequence homology, it is suggested that the GLEYA domain would predominantly contain beta sheets [ PUBMED:17870620 ]. The GLEYA domain is also found in Schizosaccharomyces pombe protein SWISSPROT , thought to be a kinetochore protein (Sim4 complex subunit), however no direct evidence for kinetochore association has been found [ PUBMED:16079914 ]. Furthermore, a global protein localisation study in S. pombe identified it as a secreted protein localized to the Golgi complex [ PUBMED:25452419 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan PA14 (CL0301), which has the following description:

This clan includes the PA14 domain and related families.

The clan contains the following 2 members:

GLEYA PA14

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(180)
Full
(930)
Representative proteomes UniProt
(1778)
RP15
(80)
RP35
(354)
RP55
(676)
RP75
(926)
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PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(180)
Full
(930)
Representative proteomes UniProt
(1778)
RP15
(80)
RP35
(354)
RP55
(676)
RP75
(926)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(180)
Full
(930)
Representative proteomes UniProt
(1778)
RP15
(80)
RP35
(354)
RP55
(676)
RP75
(926)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Linder T
Previous IDs: PA14_2;
Type: Domain
Sequence Ontology: SO:0000417
Author: Linder T , Bateman A
Number in seed: 180
Number in full: 930
Average length of the domain: 93.9 aa
Average identity of full alignment: 25 %
Average coverage of the sequence by the domain: 11.42 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 26.2 26.2
Trusted cut-off 26.2 26.2
Noise cut-off 26.1 26.1
Model length: 91
Family (HMM) version: 12
Download: download the raw HMM for this family

Species distribution

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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the GLEYA domain has been found. There are 27 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A175VNJ8 View 3D Structure Click here
P0CU04 View 3D Structure Click here
P0CU05 View 3D Structure Click here
Q7Z9I1 View 3D Structure Click here
Q92344 View 3D Structure Click here
Q9C0Y2 View 3D Structure Click here