# STOCKHOLM 1.0 #=GF ID DDAH_eukar #=GF AC PF19420.2 #=GF DE N,N dimethylarginine dimethylhydrolase, eukaryotic #=GF AU Chuguransky S;0000-0002-0520-0736 #=GF GA 23.0 23.0 #=GF NC 22.9 22.9 #=GF TC 23.0 23.0 #=GF SE Pfam-B_5784 (release 5.2) and Pfam-B_1850 (release 5.5) #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq #=GF TP Family #=GF CL CL0197 #=GF RN [1] #=GF RM 11504612 #=GF RT A novel superfamily of enzymes that catalyze the modification of #=GF RT guanidino groups. #=GF RA Shirai H, Blundell TL, Mizuguchi K; #=GF RL Trends Biochem Sci 2001;26:465-468. #=GF RN [2] #=GF RM 14675764 #=GF RT Prediction of the structure and function of AstA and AstB, the #=GF RT first two enzymes of the arginine succinyltransferase pathway of #=GF RT arginine catabolism. #=GF RA Shirai H, Mizuguchi K; #=GF RL FEBS Lett 2003;555:505-510. #=GF DR SCOP; 1jdw; fa; #=GF DR SO; 0100021; polypeptide_conserved_region; #=GF CC This family contains N(G),N(G)-dimethylarginine #=GF CC dimethylaminohydrolases (DDAH) from eukaryotes. It also includes #=GF CC arginine deiminases and DDAH from prokaryotes. These enzymes are #=GF CC involved in arginine metabolism and belong to the #=GF CC amidinotransferase (AT) superfamily as they share the alpha/beta #=GF CC propeller fold, which includes structurally important residues #=GF CC (buried hydrophobic residues, buried hydrophilic residues #=GF CC hydrogen-bonded with mainchain groups and the hallmark of three #=GF CC consecutive buried Gly residues near the C-terminus, conserved #=GF CC among its members [1,2]. #=GF SQ 3238 Q8RGP8.1/21-309 FTFN.EETAVNNHYQKK............DN.K..P...I....Q...E...IQ.NN.AL.T.E.FDNM....V.EKL..K...K...T...G...I..D..V...K....V...M.......QD......T.K.K......PH.......TP......D......S......IFPNN....WFS.T................HY.....S.....N.....T.V.....V....L.Y.P..MF.AE.N....R..RL.E..R...T..D....N.LY.DYFDET.....--.D.NL.....NV.VDYS......S..L.E..N....E..NI.F.LEG.....T.....GS.L......V......L....D...R..K........N...K....K........A.........Y..C.........S.........L.........S.........E..........R.ANEK....LL...DI.F...C......K...D.A.....G.......YK.....KI.A.F.H.S.Y..Q..........T.......V.D.E.K.R.K.P.....I..YHTN........V...M.M.A.M........G.E...N...Y......A........I......L.........C........A.D.S.I........DN..L..K.E..R.ENV..I.RE.....LK.....N.....D.D.....KEIVYI..S..E..Y..Q..V.....E...H.F..LG...N.TIEL-......INN.EN.VNIC..vMSATA..YSV.LTD..E.Q...K.N.I..I.EK.....Y..D..V..I.V.PVDV.HT.IERYGGGSARCMIAELF A6L2I9.1/17-307 FGFN.EETAANNYFQQR............TV.A..A...G....E...T...VQ.AC.AL.K.E.FRGM....V.DKL..R...A...N...G...I..E..V...I....A...V.......QD......T.P.I......PH.......TP......D......S......IFPNN....WVS.F................HD.....D.....G.....T.V.....V....L.Y.P..MF.AE.N....R..RA.E..R...R..A....D.IL.QRIESE.....GF.S.IR.....RK.VDYT......G..F.E..E....E..NR.F.LEG.....T.....GG.I......I......L....D...R..V........N...R....Y........A.........Y..A.........A.........L.........S.........Q..........R.VDEA....LF...GR.F...C......K...E.Q.....N.......FI.....PV.S.F.H.A.Y..Q..........T.......V.N.G.Q.R.L.P.....I..YHTN........V...M.M.C.V........A.T...D...Y......A........V......V.........C........L.D.S.I........DD..V..E.E..R.EQV..C.HV.....LR.....Q.....T.G.....KEIIAI..S..E..E..Q..M.....H...H.F..AG...N.MLQVE......NGK.GA.LFLV...MSQSA..YDS.LDE..G.Q...L.Q.R..L.SK.....Y..N..A..L.L.PMPI.PT.IETLGGGSARCMMAEIF A3HWB9.2/7-297 FGFN.TETAANNFYQQM............DD.R..S...G....K...E...IQ.EL.AR.K.E.FDGF....V.ALL..R...D...Q...R...V..N..V...E....V...V.......ED......T.E.N......PV.......KT......D......A......VFPNN....WFS.T................HS.....D.....G.....K.L.....I....M.Y.P..MF.SP.N....R..RL.E..R...R..K....D.LI.EMLMHK.....GY.Q.VS.....DI.LDLS......F..F.E..N....D..GQ.Y.LEG.....T.....GS.M......V......M....D...H..E........S...K....T........I.........F..A.........C.........Y.........S.........E..........R.THPF....PL...QY.V...A......E...L.L.....D.......YK.....VV.G.F.H.S.V..Q..........E.......I.E.G.V.Q.S.P.....I..YHTN........V...M.M.H.I........G.R...D...L......A........V......V.........C........L.D.S.I........PK..A..S.E..K.QLV..Q.RT.....LT.....N.....N.G.....KKVIPI..T..P..K..Q..K.....F...N.F..AG...N.MLELS......NDG.GE.KFTI...MSQTA..YDS.LNP..G.Q...I.Q.L..I.EK.....Y..T..T..I.I.SPQI.PT.IEKLGGGSARCMIAEIF C1DBM8.1/17-309 FYFD.TETAANDFFQKN............LG.E..K...R....D...V...VQ.AK.AL.I.E.FDAM....V.DLL..R...S...K...G...I..T..V...H....V...L.......QD......S.N.Q.....vMD.......TP......D......S......IFPNN....WFV.S................ME.....G.....G.....H.L.....M....L.C.P..MW.AP.N....R..RH.E..R...L..K....F.LG.QLVDLI.....GH.D.KL.....KV.SNYG.....pA..H.E..A....Q..SK.F.LEG.....T.....GA.M......I......L....D...R..V........N...M....K........A.........Y..A.........C.........L.........S.........P..........R.CDED....VF...KK.F...C......K...E.F.....G.......FK.....PV.A.F.H.G.F..Q..........T.......Y.E.G.K.R.A.A.....I..YHTN........V...M.M.A.L........G.E...A...F......A........V......V.........C........L.S.A.I........DD..L..T.E..R.AAL..V.KE.....LQ.....G.....D.G.....KEIIDV..T..E..D..Q..I.....N...N.F..AG...N.EIELC......NAD.GK.RFTI...MTKAT..YDC.LTP..E.Q...R.A.V..I.EK.....T..S..E..I.I.SPDV.KT.IETYGGGSVRCMISEIF A0A0X3AQF9.1/7-297 FGFN.QQTATNNFFQQN............DS.V..S...L....E...S...IQ.EK.AL.E.E.FRNM....V.EKL..R...K...F...G...I..N..V...L....V...V.......KD......T.E.Q......PH.......KP......D......S......IFPNN....WIS.F................HS.....D.....G.....K.V.....V....L.Y.P..MY.AK.N....R..RA.E..R...R..E....D.IL.DIIEQE.....GF.I.IN.....EI.IDYS......K..A.E..E....E..EI.F.LEG.....T.....GS.M......V......L....D...R..T........H...E....I........A.........Y..A.........A.........I.........S.........E..........R.THEE....LF...IE.F...C......E...D.L.....E.......FS.....PV.V.F.H.S.T..Q..........E.......K.E.G.K.R.Y.P.....V..YHTN........V...M.M.S.V........T.E...D...F......A........I......L.........C........K.E.S.I........QD..A..K.E..R.KLV..I.DF.....LQ.....T.....T.K.....KEVISI..S..E..E..Q..M.....N...H.F..LG...N.TLQLE......NDK.KE.RFLV...MSHTA..YQS.LNA..G.Q...I.N.A..I.NK.....Y..S..Q..I.L.VFDI.PT.IEKYGGGSVRCMLAEIF Q5NES8.1/17-303 FCFN.QETSVNNAFQNQ............LD.I..S...N....D...E...LQ.NR.VI.H.E.FENM....V.AKI..R...A...N...G...I..E..V...I....V...L.......ES......K.-.-......PN.......TP......D......A......VFPNN....WFS.Th.............liDN.....Q.....P.....Y.V.....F....I.Y.P..MY.TQ.N....R..RN.E..V...Q..V....D.KL.LEQLNK....lTT.T.NY.....KV.IDLR......-..-.G..D....Y..SK.A.LEG.....T.....GV.F......I......F....D...H..E........F...K....T........A.........Y..M.........S.........L.........S.........P..........R.ADAQ....LA...QQ.V...C......D...K.I.....G.......YK.....LV.T.F.T.S.-..-..........-.......-.Y.D.K.K.G.P.....I..YHTN........V...M.L.S.I........G.E...Y...L......A........I......V.........C........L.E.S.I........KS..A..I.E..R.ELV..I.KT.....LQ.....Q.....S.N.....KQIIDI..S..L..E..Q..M.....Y...Q.M..CG...N.VLEVK......NKA.DK.SFLI...LSQTA..YNG.FSQ..S.Q...L.A.L..I.SK.....Y..A..T..P.I.ACDI.TN.IEVVGGGSARCMLAEVF A0A1M6LZ88.1/15-305 FRKN.EQTAVNNYFQED............MD.L..K...K....V...T...IN.ER.AQ.K.E.FDAF....V.AAL..R...Q...K...G...V..N..V...V....V...V.......DD......T.A.E......PN.......TP......D......S......IFPNN....WIS.T................HA.....D.....G.....T.L.....A....L.Y.P..MY.AE.N....R..RK.E..R...R..E....D.IL.DLLEEQ.....GF.R.ID.....TI.VDYT......S..A.E..E....E..GV.F.LES.....T.....GS.M......I......L....D...R..I........H...Q....K........A.........Y..C.........A.........L.........S.........E..........R.ADED....LF...IE.F...C......E...D.F.....E.......YT.....PV.V.F.T.A.N..Q..........S.......V.D.G.K.R.M.P.....I..YHTN........V...M.M.C.V........A.E...D...F......A........V......I.........C........L.D.S.I........DD..K..K.E..R.KNV..L.DH.....LK.....Q.....D.N.....KEVIAI..T..E..E..Q..M.....H...R.F..AG...N.MLQVL......GED.DK.RFLV...MSSSA..YKS.LTD..A.Q...I.G.R..I.QA.....H..C..D..I.I.HSPL.DT.IETCGGGSARCMMAEVF G2IWP8.1/15-300 FLSN.PQTSPSNTFQKS............-D.I..D...H....Q...E...AQ.QN.AL.A.E.FDAY....A.GEL..R...R...C...G...V..D..V...L....V...I.......DD......T.V.E......PH.......TP......D......S......IFPNN....WIS.L................HA.....D.....G.....K.V.....L....L.Y.P..ME.AP.N....R..RL.E..R...N..N....K.VL.EKITEA.....-F.A.VE.....EV.IDLS......G..F.E..R....V..GQ.Y.HEG.....T.....GT.M......I......F....D...H..N........Q...K....M........A.........Y..V.........C.........F.........S.........S..........R.SSEA....VM...KA.V...S......E...T.I.....G.......YQ.....AL.W.F.H.S.-..-..........-.......V.D.R.H.G.K.P.....I..YHTN........V...M.M.C.V........G.K...T...L......A........V......V.........C........L.E.S.I........RD..R..N.E..R.TAL..V.QA.....LT.....A.....S.G.....KEIIDI..S..Y..D..Q..L.....E...H.F..AG...N.MLELS......SRD.GE.AVFA...LSRQA..WSS.LTP..E.Q...Q.T.A..I.SS.....Y..A..R..V.A.QAPI.DT.IERLGGGGARCMIAEIF A0A077EIT8.1/7-295 FGFN.AQTAVNNYFQ--............VN.D..E...S....K...E...TQ.SE.AL.R.E.FNAF....V.DKL..R...S...K...G...V..N..V...I....T...V.......KD......T.L.D......PH.......TP......D......S......IFPNN....WVS.F................HQ.....D.....G.....T.V.....I....L.Y.P..MF.AE.N....R..RL.E..R...R..S....D.IL.DQLSGK.....GL.S.IA.....AV.KDYS......V..F.E..A....E..NK.F.LEG.....T.....GS.M......I......L....D...R..E........N...K....I........A.........Y..G.........A.........V.........S.........L..........R.LDEE....IF...RK.W...C......A...E.F.....G.......YK.....PV.V.F.H.S.F..Q..........S.......V.N.G.E.R.L.P.....I..YHTN........V...M.M.C.V........G.G...D...Y......A........V......I.........C........L.D.T.I........DD..A..T.E..R.EQV..E.KA.....LT.....E.....S.H.....KEVIEI..T..E..E..Q..M.....Q...N.F..AG...N.MLEVR......NND.GH.KFLV...MSECA..YKS.LTS..R.Q...I.Q.T..I.EA.....H..A..E..I.L.YSDL.HT.IEKNGGGSARCMMAEVF A0A2S7WBH7.1/16-306 FRMN.EQTAVNNYYQEA............LD.L..K...N....A...E...IN.KK.AQ.F.E.FDTY....V.DKL..K...S...H...G...I..E..V...V....V...I.......SD......T.D.E......FD.......TP......D......A......LFPNN....WIS.F................HE.....D.....G.....T.V.....A....L.Y.P..MF.AE.N....R..RH.E..R...R..E....D.IL.DILEEK.....GF.L.IE.....NV.IDYT......S..A.E..E....E..EI.F.LEG.....T.....GS.L......L......L....D...R..V........N...K....K........A.........Y..C.........A.........L.........S.........P..........R.ADED....LF...IE.F...C......E...D.F.....E.......YT.....PV.I.F.T.A.Y..Q..........T.......V.D.G.K.R.K.P.....I..YHTN........V...M.M.C.L........A.E...T...F......A........V......I.........C........L.D.T.I........DD..K..Q.E..R.KNV..V.KH.....LK.....E.....D.R.....KHIIEI..T..E..N..Q..V.....T...H.F..AG...N.MLQVK......GTN.NE.RFLV...MSQAA..FDC.LTP..T.Q...I.Q.Q..I.TK.....H..C..K..I.I.SSSL.ET.IETCGGGSARCMMAEIF A0A1I7F7M2.1/16-306 FRMN.EQTAVNNYFQED............LE.L..Q...N....Q...E...IN.KK.AQ.E.E.FDTF....V.QKL..K...M...V...G...V..N..V...V....V...I.......QD......T.E.E......PD.......TP......D......A......IFPNN....WIS.F................HA.....N.....G.....D.V.....A....L.Y.P..MF.AE.N....R..RL.E..R...N..E....T.VL.ETLESQ.....GF.I.IN.....NV.VDYT......E..A.E..E....E..SL.F.LEG.....T.....GS.I......I......L....D...R..E........N...R....K........A.........Y..C.........A.........L.........S.........P..........R.ADED....LF...IE.F...C......E...D.F.....E.......YT.....PV.I.F.T.A.N..Q..........T.......V.D.G.E.R.K.P.....I..YHTN........V...M.M.A.I........A.E...T...F......A........V......V.........C........L.A.T.I........DD..K..K.E..R.KLV..V.NQ.....LK.....E.....N.G.....KEVIAI..T..E..G..Q..M.....Q...Q.F..AG...N.MLQVK......GSD.ER.KFLV...MSQTA..YKS.LTQ..D.Q...I.Q.K..I.EN.....H..C..T..I.L.YSDL.NT.IETCGGGSARCMLAEVF D7JFW0.1/15-305 FGFN.EETAKNNYFQQN............DN.T..A...E....A...Q...IQ.HN.AL.T.E.FRSM....V.DML..R...A...H...K...V..N..V...I....V...V.......KD......T.A.E......PH.......TP......D......S......IFPNN....WVS.F................HS.....C.....G.....R.V.....V....L.Y.P..MF.AA.N....R..RT.E..R...R..P....E.IL.DRIASE.....GF.A.IR.....EK.IDFT......G..Y.E..H....E..EK.F.LEG.....T.....GS.M......I......L....D...R..D........N...H....I........A.........Y..A.........C.........L.........S.........Q..........R.TERN....LF...EL.F...C......R...E.M.....E.......YT.....PV.A.F.H.S.F..Q..........S.......V.E.G.K.R.L.P.....I..YHTN........V...M.M.C.V........A.D...R...Y......V........I......I.........C........T.D.T.I........DD..A..A.E..R.KAV..V.DT.....IE.....K.....S.G.....KKIIEI..S..E..S..Q..M.....H...R.F..AG...N.MLQVN......NTD.GD.KFLV...MSDTA..YRS.LRP..E.Q...V.A.A..I.EQ.....Y..N..K..I.L.HPAI.PT.IEHYGGGSARCMMAEIF A0A0A2EU04.1/7-297 FGFN.EETAKNNYFQQN............DN.T..A...E....S...Q...IQ.QN.AL.K.E.FTAM....V.ETL..R...G...K...K...I..N..V...I....V...E.......KD......T.I.S......PH.......TP......D......S......IFPNN....WVS.F................HS.....C.....G.....K.V.....V....L.Y.P..MF.AE.N....R..RT.E..R...R..P....D.IL.ERIKSE.....GF.S.IN.....QN.IDFS......G..Y.E..H....Q..NK.F.LEG.....T.....GS.M......I......L....D...R..D........N...H....I........A.........Y..A.........C.........I.........S.........Q..........R.TDKN....LF...EL.F...C......R...E.L.....E.......YT.....PV.S.F.S.S.F..Q..........T.......V.E.D.K.R.L.P.....I..YHTN........V...M.M.C.V........A.D...H...Y......V........V......I.........C........L.D.T.I........DN..A..D.E..R.KMV..M.ES.....IE.....K.....S.G.....KKIVEI..T..E..K..Q..M.....H...L.F..AG...N.MLQVN......NIE.GE.KFLV...MSQTA..YES.LTK..E.Q...V.E.T..I.ES.....F..N..A..I.I.HVPI.PT.IERYGGGSARCMMAEVF A0A1W2AY78.1/7-298 FRFN.AETAVNNYFQNE............TD.E..S...S....E...S...IQ.QK.AL.N.E.FQAM....V.SKL..R...E...H...G...V..N..V...I....T...I.......KD......T.L.E......PH.......TP......D......S......IFPNN....WIS.F................HE.....N.....A.....T.I.....A....L.Y.P..MF.AQ.N....R..RD.E..R...R..E....D.DVlDLLGEK.....GF.D.TE.....EV.MDYT......S..A.E..E....D..EI.Y.LEG.....T.....GS.I......I......L....D...R..E........N...G....I........A.........Y..A.........A.........I.........S.........P..........R.TDEE....LF...LE.F...C......E...D.F.....E.......YT.....PV.I.F.E.A.N..Q..........T.......V.D.G.E.R.M.Q.....I..YHTN........V...M.M.C.V........A.D...E...Y......A........V......I.........C........L.D.T.I........DD..K..S.E..K.KNV..V.AS.....LK.....E.....S.G.....KEIISI..T..E..E..Q..M.....N...Q.F..AG...N.MLQVG......GMG.SS.KYLV...MSQTA..YNS.LTP..E.Q...V.E.Q..I.EK.....F..N..K..I.I.SVDI.NT.IETLGGGSARCMMAEVF W2URJ8.1/17-307 FRMN.EQTAVNNYFQTT............ID.L..Q...N....N...A...IN.RK.AT.E.E.FDVF....V.EKL..R...E...V...G...V..N..V...I....V...I.......DD......T.T.E......PN.......TP......D......S......IFPNN....WIS.F................HQ.....E.....G.....T.V.....A....V.Y.P..MF.AE.N....R..RF.E..R...R..E....D.VL.EKIEEK.....GF.V.ID.....NV.VDYT......S..A.E..E....E..GV.F.LEG.....T.....GS.L......I......L....D...R..V........N...R....K........A.........Y..C.........A.........L.........S.........P..........R.ADEE....LV...IE.F...C......E...D.F.....E.......YT.....PV.I.F.T.A.N..Q..........T.......V.N.G.K.R.L.P.....I..YHTN........V...M.M.A.I........G.D...T...F......A........V......I.........C........L.D.T.I........DD..K..K.E..R.KNV..A.KH.....LS.....E.....D.G.....KEIISI..T..E..A..Q..M.....Q...Q.F..AG...N.MLQVK......GND.DR.SYLV...MSEAA..YKS.LTP..N.Q...I.S.K..I.EA.....H..S..N..I.L.YSSL.HT.IETCGGGSARCMMAEVF I4A2C7.1/18-308 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