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63  structures 5135  species 0  interactions 13670  sequences 262  architectures

Family: BD-FAE (PF20434)

Summary: BD-FAE

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The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

BD-FAE Provide feedback

This family represents a novel bifunctional feruloyl and acetyl xylan esterase (BD-FAE, previously known as bifunctional carbohydrate esterase (CE)), which is active on complex natural xylans and was identified as the basis of a monophyletic clade gathering all homologs identified in PULs (polysaccharide utilisation loci) predicted to act on xylan. It adopts an alpha-beta-hydrolase fold with the catalytic triad Ser-Asp-His [1]. This new family of proteins is a new candidate for biomass processing due to its capacity to remove ferulic acid and acetic acid from natural corn and birchwood xylan substrates [1].

Literature references

  1. Hameleers L, Penttinen L, Ikonen M, Jaillot L, Faure R, Terrapon N, Deuss PJ, Hakulinen N, Master ER, Jurak E;, Biotechnol Biofuels. 2021;14:127.: Polysaccharide utilization loci-driven enzyme discovery reveals BD-FAE: a bifunctional feruloyl and acetyl xylan esterase active on complex natural xylans. PUBMED:34059129 EPMC:34059129


Internal database links

This tab holds annotation information from the InterPro database.

No InterPro data for this Pfam family.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(75)
Full
(13670)
Representative proteomes UniProt
(54625)
RP15
(1818)
RP35
(6471)
RP55
(13652)
RP75
(23587)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(75)
Full
(13670)
Representative proteomes UniProt
(54625)
RP15
(1818)
RP35
(6471)
RP55
(13652)
RP75
(23587)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(75)
Full
(13670)
Representative proteomes UniProt
(54625)
RP15
(1818)
RP35
(6471)
RP55
(13652)
RP75
(23587)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

This family is new in this Pfam release.

Seed source: Hameleers Lisanne
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Hameleers L , Faure R , Terrapon N , Hakulinen N , Deuss P , Master E , Jurak E , Mistry J , Chuguransky S
Number in seed: 75
Number in full: 13670
Average length of the domain: 178.7 aa
Average identity of full alignment: 21 %
Average coverage of the sequence by the domain: 51.37 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 26.8 26.8
Trusted cut-off 26.8 26.8
Noise cut-off 26.7 26.7
Model length: 215
Family (HMM) version: 1
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the BD-FAE domain has been found. There are 63 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A044RJF2 View 3D Structure Click here
A0A077ZCR2 View 3D Structure Click here
A0A077ZDD9 View 3D Structure Click here
A0A0D2DR66 View 3D Structure Click here
A0A0D2E8H8 View 3D Structure Click here
A0A0D2EY55 View 3D Structure Click here
A0A0D2GBK7 View 3D Structure Click here
A0A0H3GNR0 View 3D Structure Click here
A0A0K0E2A5 View 3D Structure Click here
A0A0K0IQF9 View 3D Structure Click here
A0A0R0EHV8 View 3D Structure Click here
A0A0R4J5X2 View 3D Structure Click here
A0A175VZ24 View 3D Structure Click here
A0A175W6F9 View 3D Structure Click here
A0A1C1CLR8 View 3D Structure Click here
A0A1C1CZJ1 View 3D Structure Click here
A0A1D6EGD3 View 3D Structure Click here
A0A1D6FL00 View 3D Structure Click here
A0A1D6GVU0 View 3D Structure Click here
A0A1D6HZD9 View 3D Structure Click here
A0A1D6J2H6 View 3D Structure Click here
A0A1D6MDL4 View 3D Structure Click here
A0A2I1BSV9 View 3D Structure Click here
A4HWD4 View 3D Structure Click here
A4HWD5 View 3D Structure Click here
A5DNX8 View 3D Structure Click here
A5PMM7 View 3D Structure Click here
A7TJ85 View 3D Structure Click here
B4FFC7 View 3D Structure Click here
B5XB27 View 3D Structure Click here
D5EV35 View 3D Structure Click here
I1MMN5 View 3D Structure Click here
I6Y9F7 View 3D Structure Click here
K0ETP0 View 3D Structure Click here
K0EY69 View 3D Structure Click here
K0EZJ5 View 3D Structure Click here
K7L956 View 3D Structure Click here
M0RC77 View 3D Structure Click here
O06832 View 3D Structure Click here
P96402 View 3D Structure Click here