Powering down the Pfam website
On October 5th, we began redirecting traffic from Pfam (pfam.xfam.org) to InterPro (www.ebi.ac.uk/interpro). The Pfam website will remain available at pfam-legacy.xfam.org until January 2023, when it will be decommissioned. You can read more about the sunset period in our blog post.

Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
219  structures 583  species 0  interactions 63967  sequences 3437  architectures

Family: TSP_1 (PF00090)

Summary: Thrombospondin type 1 domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Thrombospondin". More...

Thrombospondin Edit Wikipedia article

Thrombospondins are secreted proteins with the ability to inhibit angiogenesis. Overexpression of TSPs in tumor cells results in reduced angiogenesis. Inhibitory peptides and fragments of TSP1 bind to CD36, leading to the expression of FasL, which activates the expression of Fas. This leads to the activation of caspases and apoptosis of the cell.

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Thrombospondin type 1 domain Provide feedback

No Pfam abstract.

Literature references

  1. Bork P; , FEBS Lett 1993;327:125-130.: The modular architecture of a new family of growth regulators related to connective tissue growth factor. PUBMED:7687569 EPMC:7687569

Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000884

Thrombospondins are multimeric multidomain glycoproteins that function at cell surfaces and in the extracellular matrix milieu. They act as regulators of cell interactions in vertebrates. They are divided into two subfamilies, A and B, according to their overall molecular organisation. The subgroup A proteins TSP-1 and -2 contain an N-terminal domain, a VWFC domain, three TSP1 repeats, three EGF-like domains, TSP3 repeats and a C-terminal domain. They are assembled as trimer. The subgroup B thrombospondins, designated TSP-3, -4, and COMP (cartilage oligomeric matrix protein, also designated TSP-5) are distinct in that they contain unique N-terminal regions, lack the VWFC domain and TSP1 repeats, contain four copies of EGF-like domains, and are assembled as pentamers [ PUBMED:11687483 ]. EGF, TSP3 repeats and the C-terminal domain are thus the hallmark of a thrombospondin.

This repeat was first described in 1986 by Lawler and Hynes [ PUBMED:2430973 ]. It was found in the thrombospondin protein where it is repeated 3 times. Now a number of proteins involved in the complement pathway (properdin, C6, C7, C8A, C8B, C9) [ PUBMED:2459396 ] as well as extracellular matrix protein like mindin, F-spondin [ PUBMED:10409509 ], SCO-spondin and even the circumsporozoite surface protein 2 and TRAP proteins of Plasmodium [ PUBMED:10508153 , PUBMED:1501644 ] contain one or more instance of this repeat. It has been involved in cell-cell interaction, inhibition of angiogenesis [ PUBMED:10500044 ] and apoptosis [ PUBMED:9135017 ].

The intron-exon organisation of the properdin gene confirms the hypothesis that the repeat might have evolved by a process involving exon shuffling [ PUBMED:1417780 ]. A study of properdin structure provides some information about the structure of the thrombospondin type I repeat [ PUBMED:1868073 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Pfam Clan

This family is a member of clan TSP1 (CL0692), which has the following description:

This superfamiy includes the TSP1 domain and its variants.

The clan contains the following 4 members:



We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Representative proteomes UniProt
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

Representative proteomes UniProt

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

Representative proteomes UniProt
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Published_alignment
Previous IDs: tsp_1;
Type: Domain
Sequence Ontology: SO:0000417
Author: Sonnhammer ELL
Number in seed: 24
Number in full: 63967
Average length of the domain: 49.5 aa
Average identity of full alignment: 34 %
Average coverage of the sequence by the domain: 10.76 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.6 12.0
Trusted cut-off 21.6 12.0
Noise cut-off 21.5 11.9
Model length: 49
Family (HMM) version: 22
Download: download the raw HMM for this family

Species distribution

Sunburst controls


Weight segments by...

Change the size of the sunburst


Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls


The tree shows the occurrence of this domain across different species. More...


Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.


For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the TSP_1 domain has been found. There are 219 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

Loading structure mapping...

AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A044RPV5 View 3D Structure Click here
A0A044RYL9 View 3D Structure Click here
A0A044SEU3 View 3D Structure Click here
A0A044SKR7 View 3D Structure Click here
A0A044SLU3 View 3D Structure Click here
A0A044SPT6 View 3D Structure Click here
A0A044SUW2 View 3D Structure Click here
A0A044SXR6 View 3D Structure Click here
A0A044T836 View 3D Structure Click here
A0A044TEA1 View 3D Structure Click here
A0A044U6P4 View 3D Structure Click here
A0A044U6T0 View 3D Structure Click here
A0A044UTN8 View 3D Structure Click here
A0A077YYY8 View 3D Structure Click here
A0A077Z1T3 View 3D Structure Click here
A0A077Z213 View 3D Structure Click here
A0A077Z271 View 3D Structure Click here
A0A077Z2S6 View 3D Structure Click here
A0A077Z3K5 View 3D Structure Click here
A0A077Z3L6 View 3D Structure Click here
A0A077Z7F7 View 3D Structure Click here
A0A077ZA16 View 3D Structure Click here
A0A077ZB48 View 3D Structure Click here
A0A077ZDE6 View 3D Structure Click here
A0A0G2JV24 View 3D Structure Click here
A0A0G2K229 View 3D Structure Click here
A0A0G2K7X7 View 3D Structure Click here
A0A0G2KI55 View 3D Structure Click here
A0A0G2KPG1 View 3D Structure Click here
A0A0J9XN59 View 3D Structure Click here
A0A0K0DVL6 View 3D Structure Click here
A0A0K0E1H2 View 3D Structure Click here
A0A0K0E1W7 View 3D Structure Click here
A0A0K0E4D6 View 3D Structure Click here
A0A0K0E5T7 View 3D Structure Click here
A0A0K0E8Q0 View 3D Structure Click here
A0A0K0E9V4 View 3D Structure Click here
A0A0K0EBK8 View 3D Structure Click here
A0A0K0EDS6 View 3D Structure Click here
A0A0K0EHY3 View 3D Structure Click here